Streamline Your Lab: Why AutoDimer is the Future of PCR Multiplexing

AutoDimer is a specialized bioinformatics software package developed by the National Institute of Standards and Technology (NIST) to rapidly screen preselected PCR primers for potential cross-reactivity. It is widely used to optimize multiplex PCR assay design. When multiple primer sets are pooled in a single reaction tube, they often bind to one another instead of the target DNA. AutoDimer prevents this by analyzing short DNA oligomers (typically fewer than 30 nucleotides) for problematic structures before you begin wet-lab testing.

The software is accessible via the NIST DNA Analysis Tools portal. Key Capabilities of AutoDimer AutoDimer | NIST